Transfer of Pectobacterium carotovorum subsp. carotovorum strains isolated from potatoes grown at high altitudes to Pectobacterium peruviense sp. nov.

Małgorzata Waleron , Agnieszka Misztak , Michał Waleron , Martyna Franczuk , Bartosz Wielgomas , Krzysztof Waleron

Abstract

tSeven Gram-negative, rod-shaped pectinolytic bacteria strains designated as IFB5227, IFB5228, IFB5229,IFB5230, IFB5231, IFB5232, IFB5636, isolated from potato tubers cultivated in Peru at high altitude(2400–3800 m) were subjected to polyphasic analyses that revealed their distinctiveness from the otherPectobacterium species. Phylogenetic analyses based on five housekeeping genes (gyrA, recA, recN, rpoAand rpoS) clearly showed strains separateness, simultaneously indicating Pectobacterium atrosepticum,Pectobacterium wasabiae, Pectobacterium parmentieri and Pectobacterium betavasculorum as the closestrelatives. In silico DNA–DNA hybridization of strain IFB5232Twith other Pectobacterium type strainsrevealed significant drop in DDH value below 70%, which is a prerequisite to distinguish Pectobacteriumperuviense. The ANI values supported the proposition of delineation of the P. peruviense. Genetic REP-PCRfingerprint and detailed MALDI-TOF MS proteomic profile sealed the individuality of the studied strains.However, phenotypic assays do not indicate immense differences. Provided results of analyses performed for seven Peruvian strains are the basis for novel speciesdistinction and reclassification of the strains IFB5227-5232 and IFB5636, previously classified as Pec-tobacterium carotovorum subsp. carotovorum. Here, we propose to establish the IFB5232 isolate as a typestrain (=PCM2893T= LMG30269T= SCRI179T) with the name Pectobacterium peruviense sp. nov.
Author Małgorzata Waleron KB
Małgorzata Waleron,,
- Department of Biotechnology
, Agnieszka Misztak MWB UG i GUMed
Agnieszka Misztak,,
- Intercollegiate Faculty of Biotechnology UG
, Michał Waleron MWB UG i GUMed
Michał Waleron,,
- Intercollegiate Faculty of Biotechnology UG
, Martyna Franczuk
Martyna Franczuk,,
-
, Bartosz Wielgomas
Bartosz Wielgomas,,
-
, Krzysztof Waleron
Krzysztof Waleron,,
-
Journal seriesSystematic and Applied Microbiology, ISSN 0723-2020
Issue year2018
Vol41
No2
Pages85-93
Publication size in sheets0.5
Keywords in EnglishMLSA, ANI, is DNA-DNA hybridization, FAME, MALDI-TOF MS, potato pathogen
DOIDOI:10.1016/j.syapm.2017.11.005
URL https://doi.org/10.1016/j.syapm.2017.11.005
Languageen angielski
Score (nominal)35
ScoreMinisterial score = 30.0, 01-06-2018, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 01-06-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 3.931 (2) - 2016=3.739 (5)
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