Molecular detection of bacteria associated withSerendipitasp., a mycorrhizal fungus from the orchidStanhopea connataKlotzsch in southern Ecuador

Alzbeta Novotna , J. P. Suárez

Abstract

We examined the bacterial community associated with an orchid mycorrhizal fungus from the Neotropical region. Living hyphae of Serendipita sp., isolated from roots of Stanhopea connata, were screened using molecular tools to identify associated prokaryotes. In total, nine distinctive bacterial OTUs were detected belonging to phyla Firmicutes, Proteobacteria and Acidobacteria. Within Firmicutes the genera Bacillus, Gemella, Staphylococcus and Streptococcus were detected. Other genera such as Paracoccus, Achromobacter from Proteobacteria and Acidobacter from Acidobacteria were also revealed. Additionally, one OTU with close similarity to Neisseriaceae of the Betaproteobacteria was detected. Further investigations, eg fluorescent or electron microscopy, are necessary to determine whether the uncovered bacteria are situated inside or outside the fungal hyphae.
Author Alzbeta Novotna (FB / DPTNC)
Alzbeta Novotna ,,
- Department of Plant Taxonomy and Nature Conservation
, J. P. Suárez
J. P. Suárez,,
-
Journal seriesActa Botanica Gallica, ISSN 1253-8078, (A 15 pkt)
Issue year2018
Vol165
No2
Pages307-313
Publication size in sheets0.5
Keywords in Englishbacteria, molecular detection, mycorrhiza, Neotropics, Orchidaceae, Serendipitaceae
DOIDOI:10.1080/23818107.2018.1436087
Languageen angielski
Score (nominal)15
ScoreMinisterial score = 15.0, 03-11-2018, ArticleFromJournal
Ministerial score (2013-2016) = 15.0, 03-11-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 0.871 (2) - 2016=0.542 (5)
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